Thermo Fisher Scientific p21WAF1 (Tumor Suppressor Protein) Monoclonal Antibody (AC8)
p21WAF1 단백질을 인식하는 AC8 클론 단클론 항체. Western blot, ICC/IF, ELISA, IP에 적합. 인간 시료에 반응하며 마우스 및 랫과 반응하지 않음. 단백질 A/G 정제, PBS 용액 형태로 제공. 종양 억제 단백질 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 1–5 µg/mL |
| Immunoprecipitation (IP) | 1–2 µg per 500 µg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AC8 |
| Immunogen | Recombinant full-length human CDKN1A protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse or Rat.
Target Information
Protein p21 is a tumor suppressor protein that inhibits cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. It plays a critical role in the cellular response to genotoxic stress and is a major transcriptional target of p53.
In the nucleus, p21 binds and inhibits Cdk1 and Cdk2, blocking cell cycle progression from G1 to S phase or G2 to mitosis after DNA damage, allowing DNA repair.
In the cytoplasm, p21 exhibits anti-apoptotic effects by binding and inhibiting caspase 3, ASK1, and JNK.
p21 has a dual role in carcinogenesis, acting both as a tumor suppressor and as an oncogene depending on context.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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