
Thermo Fisher Scientific p21WAF1 (Tumor Suppressor Protein) Monoclonal Antibody (AC8)
Human p21WAF1 종양 억제 단백질을 인식하는 마우스 단클론 항체로, WB, ICC/IF, ELISA, IP에 적합. p53에 의해 전사 조절되는 CDK 억제 단백질 연구용. Protein A/G 정제, 액상 형태, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 1–5 µg/mL |
| Immunoprecipitation (IP) | 1–2 µg per 500 µg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AC8 |
| Immunogen | Recombinant full-length human CDKN1A protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse or Rat.
Target Information
p21 (CDKN1A) is a tumor suppressor protein that inhibits cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. It plays a key role in the cellular response to genotoxic stress and acts as a major transcriptional target of p53.
In the nucleus, p21 binds and inhibits CDK1 and CDK2, blocking the transition from G1 to S phase or G2 to mitosis after DNA damage, allowing DNA repair.
In the cytoplasm, p21 exhibits anti-apoptotic effects by binding and inhibiting caspase 3 and apoptotic kinases ASK1 and JNK.
p21 shows dual roles in carcinogenesis, functioning as both a tumor suppressor and an oncogene depending on the cellular context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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