Thermo Fisher Scientific p21WAF1 (Tumor Suppressor Protein) Recombinant Rabbit Monoclonal Antibody (CIP1, 4377R)
p21WAF1 단백질을 표적으로 하는 재조합 토끼 단클론 항체로, 인간 시료에 반응하며 WB, IHC, Peptide Array 실험에 적합. p53에 의해 활성화되는 종양 억제 단백질 검출용으로 사용. 고순도 Protein A/G 정제, 액상 형태로 안정적 보관 가능.
- 카탈로그번호
- 1026-RBM10-P0
- 판매단위
- pk
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Thermo Fisher Scientific p21WAF1 (Tumor Suppressor Protein) Recombinant Rabbit Monoclonal Antibody (CIP1, 4377R)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CIP1, 4377R |
| Immunogen | Synthetic peptide corresponding to p21 residues within aa1–100 of p21 was used as an immunogen. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53.
p21 plays a crucial role in cellular response to genotoxic stress and is a major transcriptional target of p53.
In the nucleus, p21 binds and inhibits Cdk1 and Cdk2, blocking transition from G1 to S phase or G2 to mitosis after DNA damage, allowing DNA repair.
In the cytoplasm, p21 exhibits anti-apoptotic effects by inhibiting caspase 3 and apoptotic kinases ASK1 and JNK.
p21 has dual functions in carcinogenesis, acting both as a tumor suppressor and as an oncogene depending on cellular context.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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