Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), eBioscience
Mouse EpCAM(CD326) 인식 Rat IgG2a 단일클론 항체로, Flow Cytometry, IHC, IP 등 다양한 응용 가능. 0.5 mg/mL 액상 형태, Affinity chromatography 정제, 4°C 보관. EpCAM 관련 종양 및 세포 접착 연구용.
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Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 21 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 3 |
| Immunocytochemistry (ICC/IF) | - | 11 |
| Flow Cytometry (Flow) | 0.125 µg/test | 84 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| ChIP assay (ChIP) | - | 1 |
| Affinity Purification (AP) | - | 1 |
| In vitro Assay (IV) | - | 1 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G8.8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_953624 |
Available Conjugated Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation available
Product Specific Information
The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. Generated using the TE-71 thymic epithelial cell line as immunogen, CD326 is expressed on most epithelial cells and considered a pan-carcinoma antigen. It mediates calcium-independent, homophilic cell-cell adhesion and may function as a growth factor receptor.
CD326 interacts with LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation, playing an important role in tumor biology.
Applications Reported:
- Flow cytometric analysis
- Immunoprecipitation
- Immunohistology of frozen tissue sections
Applications Tested:
Tested by flow cytometric analysis of TE-71 murine thymic epithelial cells at ≤0.125 µg/test. A test is defined as staining a cell sample in 100 µL volume. Cell number may range from 10⁵ to 10⁸ cells/test. Antibody titration recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a calcium-independent adhesion molecule and influences cell cycle, proliferation, and metabolism via induction of c-myc, cyclin A, and E.
Ep-CAM overexpression is associated with increased epithelial proliferation and serves as a marker for epithelial-derived tumors. It is expressed on baso-lateral surfaces of most simple epithelia and carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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