Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Conjugates

• Alexa Fluor 488, 532, 561, 660, 700
• APC
• Biotin
• Brilliant Violet 421, 605, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2). Cross-reactivity with other proteins has not been determined. Foxp3 (49–55 kDa) is a member of the forkhead/winged-helix transcriptional regulator family and is defective in scurfy (sf) mice. It is highly expressed in CD4⁺CD25⁺ regulatory T cells (Treg) and induces a Treg phenotype when expressed in CD4⁺CD25⁻ cells.

Immunoblotting with FJK-16s antibody maps the epitope to amino acids 75–125 of mouse Foxp3. In humans, this region is alternatively spliced. Both isoforms are present in CD4⁺CD25⁺ lymphocytes, but not observed in mouse splenocytes, suggesting species differences in regulation.

Applications Reported: Western blotting, IHC (frozen and paraffin-embedded sections).
Applications Tested: WB (1–5 µg/mL) and IHC (≤5 µg/mL). Titrate for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is 381 amino acids long and expressed at high levels in CD25⁺CD4⁺ regulatory T cells. FOXP3 acts as a master regulator of Treg cells. Mutations in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans. Multiple transcript variants encoding different isoforms exist. In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.