Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eBioscience
FOXP3 단백질을 인식하는 FJK-16s 단클론 항체로, 마우스, 랫, 개, 돼지 등 다양한 종에 반응합니다. Western blot, IHC, Flow cytometry 등에 사용 가능하며, Treg 세포 연구에 적합합니다. 액상 형태로 제공되며, 4°C에서 보관합니다.
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- 14-5773-xx (4개 옵션)
- 판매단위
- pk
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | 26 |
| Immunohistochemistry (IHC) | - | 183 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | 32 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | 10 |
| Immunocytochemistry (ICC/IF) | - | 32 |
| Flow Cytometry (Flow) | - | 500 |
| ELISA | - | 1 |
| Immunoprecipitation (IP) | - | 3 |
| ChIP assay (ChIP) | - | 1 |
| Functional Assay | - | 1 |
| In Situ Hybridization (ISH) | - | 1 |
| Miscellaneous (PubMed) | - | 6 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Cat, Mouse, Pig, Rat |
| Published Species | Cynomolgus monkey, Dog, Horse, Human, Mouse, Pig, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FJK-16s |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865133 |
Additional Available Conjugates
- Alexa Fluor 488, 532, 561, 660, 700
- APC
- Biotin
- Brilliant Violet 421, 605, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2). Cross-reactivity with other proteins has not been determined. Foxp3 (49–55 kDa) is a member of the forkhead/winged-helix transcriptional regulator family and is defective in scurfy (sf) mice. It is highly expressed in CD4⁺CD25⁺ regulatory T cells (Treg) and induces a Treg phenotype when expressed in CD4⁺CD25⁻ cells.
Immunoblotting with FJK-16s antibody maps the epitope to amino acids 75–125 of mouse Foxp3. In humans, this region is alternatively spliced. Both isoforms are present in CD4⁺CD25⁺ lymphocytes, but not observed in mouse splenocytes, suggesting species differences in regulation.
Applications Reported: Western blotting, IHC (frozen and paraffin-embedded sections).
Applications Tested: WB (1–5 µg/mL) and IHC (≤5 µg/mL). Titrate for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is 381 amino acids long and expressed at high levels in CD25⁺CD4⁺ regulatory T cells. FOXP3 acts as a master regulator of Treg cells. Mutations in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans. Multiple transcript variants encoding different isoforms exist. In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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