
Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
DyLight 550 형광을 결합한 Donkey anti-Mouse IgG (H+L) 교차흡착 2차 항체로, WB, IHC, ICC, Flow 등 다양한 면역실험에 적합. 최소한의 교차반응성과 높은 특이성 제공. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgG-heavy and light chain |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556747 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, human, rabbit, rat, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, goat, human, rabbit, rat, and sheep.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of target proteins. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification. Most are produced by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (such as immunoaffinity chromatography, antibody fragmentation, or label conjugation) to ensure high specificity.
Additional Formats Available
- DyLight 488
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- HRP
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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