Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488

Applications

Tested Dilution

Western Blot (WB)

1:1,000-1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

1:40-1:400

Immunocytochemistry (ICC/IF)

1:40-1:500

Flow Cytometry (Flow)

1:50-1:200

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Mouse IgG-heavy and light chain

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

View additional formats

• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Donkey/)
• [DyLight 594](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Donkey/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Donkey/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Donkey/)
• [DyLight 755](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Donkey/)
• [DyLight 800](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556746

Target

IgG

Cross Adsorption

Against bovine, chicken, goat, human, rabbit, rat and sheep

Antibody Form

Whole Antibody

Product Specific Information

This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, goat, human, rabbit, rat and sheep.

For Western blot applications, 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.