Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 650
DyLight 650 형광 표지를 가진 Donkey anti-Mouse IgG (H+L) 교차흡착 2차 항체로, WB, IHC, ICC, Flow 등 다양한 응용에 사용 가능. 낮은 비특이 반응성과 높은 신호 감도로 연구용에 적합. 항원 친화 크로마토그래피로 정제됨.
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Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 650
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgG-heavy and light chain |
| Conjugate | DyLight™ 650 |
| Excitation/Emission Max | 651/673 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556749 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, human, rabbit, rat, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, goat, human, rabbit, rat, and sheep.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility enabling use with various detection systems (HRP, AP, fluorescence). They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
Additional Formats
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 680
- DyLight 755
- DyLight 800
- HRP
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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