Thermo Fisher Scientific Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 755
DyLight 755 형광 표지를 가진 Rabbit anti-Mouse IgG (H+L) 교차흡착 2차 항체로, 인간 및 랫트 반응 최소화. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 고순도 항원 친화 크로마토그래피 정제.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgG-heavy and light chain |
| Conjugate | DyLight™ 755 |
| Excitation / Emission Max | 760 / 789 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556743 |
| Target | IgG |
| Cross Adsorption | Against human and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimum reactivity to human and rat.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility by enabling the use of various detection systems (e.g., HRP, AP, fluorescence) and greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most secondary antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species, then further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
Additional Formats Available
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor 680
- AP
- Biotin
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- DyLight 800
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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