Thermo Fisher Scientific Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 650
DyLight 650 형광으로 결합된 Rabbit anti-Mouse IgG (H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등에 적합합니다. 인간 및 랫트에 대한 교차 반응이 최소화된 Cross-Adsorbed 제품입니다. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 신뢰성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgG-heavy and light chain |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556741 |
| Target | IgG |
| Cross Adsorption | Against human and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimum reactivity to human and rat.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of their targets.
Secondary antibodies enhance assay versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
Available Conjugate Formats
- Alexa Fluor 350, 488, 555, 568, 594, 633, 647, 680
- AP, Biotin, DyLight 488, 550, 680, 755, 800
- FITC, HRP, TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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