Thermo Fisher Scientific CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience
인간 CD62E(E-selectin)을 인식하는 P2H3 클론 단일클론 항체로, PE 형광표지되어 있습니다. 흐름세포분석(Flow Cytometry)에 최적화되어 있으며, 염증 시 세포 부착 관련 연구에 활용됩니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 12-0627-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience
Thermo Fisher Scientific CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 7 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P2H3 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11217891 |
Product Specific Information
The P2H3 monoclonal antibody reacts with human CD62E, a 97–115 kDa member of the selectin family. CD62E (E-selectin or ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines such as TNFα and IL-1β.
Induced expression of CD62E during inflammatory conditions mediates leukocyte rolling and initial neutrophil-endothelium interaction.
This antibody inhibits cellular adhesion to cytokine-activated endothelial cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry with TNFα-activated HUVEC cells. Recommended use at 5 µL (0.5 µg) per test (100 µL final volume).
Cell number may range from 10⁵ to 10⁸ cells/test.
Optical Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Leukocyte adhesion to endothelium occurs during inflammation and involves selectin and immunoglobulin superfamily receptors.
E-selectin (CD62E) is expressed by endothelial cells only after activation with proinflammatory cytokines.
Induced by IL-1, TNFα, and bacterial lipopolysaccharides through NFκB-dependent transcription.
E-selectin is associated with vascular endothelium in various inflammatory responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.