Thermo Fisher Scientific CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience
인간 CD62E(E-selectin)을 인식하는 P2H3 클론 단일클론 항체로, PE 형광표지되어 있습니다. 흐름세포분석(Flow Cytometry)에 최적화되어 있으며, 염증 시 세포 부착 관련 연구에 활용됩니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 7 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P2H3 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11217891 |
Product Specific Information
The P2H3 monoclonal antibody reacts with human CD62E, a 97–115 kDa member of the selectin family. CD62E (E-selectin or ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines such as TNFα and IL-1β.
Induced expression of CD62E during inflammatory conditions mediates leukocyte rolling and initial neutrophil-endothelium interaction.
This antibody inhibits cellular adhesion to cytokine-activated endothelial cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry with TNFα-activated HUVEC cells. Recommended use at 5 µL (0.5 µg) per test (100 µL final volume).
Cell number may range from 10⁵ to 10⁸ cells/test.
Optical Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Leukocyte adhesion to endothelium occurs during inflammation and involves selectin and immunoglobulin superfamily receptors.
E-selectin (CD62E) is expressed by endothelial cells only after activation with proinflammatory cytokines.
Induced by IL-1, TNFα, and bacterial lipopolysaccharides through NFκB-dependent transcription.
E-selectin is associated with vascular endothelium in various inflammatory responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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