Thermo Fisher Scientific CD61 (Integrin beta 3) Monoclonal Antibody (VI-PL2), PE, eBioscience

Thermo Fisher Scientific CD61 (Integrin beta 3) Monoclonal Antibody (VI-PL2), PE, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test
• View 6 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• FITC
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 645
• Request custom conjugation

Product Specific Information

The VI-PL2 monoclonal antibody reacts with human CD61, also known as integrin beta 3 (GPIIIa).
CD61 is a 90–110 kDa member of the beta integrin family expressed by leukocytes, platelets, endothelial, and smooth muscle cells. It forms non-covalent complexes with alpha integrins CD41 and CD51, resulting in αIIbβ3 (CD41/CD61) and αvβ3 (CD51/CD61) heterodimers. These complexes mediate cellular adhesion, trafficking, proliferation, and differentiation.

• CD41/CD61 integrin: involved in platelet aggregation; binds fibrinogen, von Willebrand Factor (vWF), and fibronectin.
• CD51/CD61 integrin: binds vitronectin, fibronectin, vWF, and fibrinogen; regulates migration and survival of angiogenic endothelial cells.

This antibody cross-reacts with canine, baboon, rhesus, and cynomolgus monkey species.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry at 5 µL (0.06 µg) per test.
A test = amount of antibody staining cells in 100 µL final volume (typically 10⁵–10⁸ cells/test).
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD61 (GPIIIa, ITGB3) is a glycoprotein expressed on megakaryocytes, platelets, and precursors. It acts as a receptor for fibrinogen, fibronectin, von Willebrand factor, and vitronectin, playing a key role in platelet aggregation and cell adhesion.

• Molecular Weight: ~105 kDa
• Associations: Forms complexes with αIIb (CD41) or αV (CD51) integrins
• Expression: Platelets, megakaryocytes, endothelial cells, monocytes, osteoclasts
• Functions: Cell adhesion and signaling
• Associated Diseases: Glanzmann Thrombasthenia, Platelet type-16 Bleeding Disorder

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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