Thermo Fisher Scientific Resistin Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• 권장 농도: 0.1–0.2 µg/mL

ELISA

• 권장 농도: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SSMPLCPIDE AIDKKIKQDF NSLFPNAIKN IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI DWTAARCCKL QVAS

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine Resistin. Anti-Murine Resistin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mResistin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Resistin (500-P182G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mResistin.

Western Blot:
To detect Murine Resistin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine Resistin is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P182GBT-1MG will be provided as 2 × 500 µg.

Target Information

Adipose tissue serves as an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin.
Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine specifically secreted by adipocytes. It appears to have paracrine roles and a suggested role in type 2 diabetes. In obese, diabetic mice, serum resistin levels are increased and antagonize insulin action. Resistin causes insulin resistance and glucose intolerance in mice.
Resistin gene expression requires C/EBP alpha, and coactivators CBP and p300 acetylate histones at the resistin promoter, activating chromatin for transcription. PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.