Thermo Fisher Scientific Resistin Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SSKTLCSMEE AINERIQEVA GSLIFRAISS IGLECQSVTS RGDLATCPRG FAVTGCTCGS ACGSWDVRAE TTCHCQCAGM DWTGARCCRV QP

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Resistin. Anti-Human Resistin-specific antibody was purified by affinity chromatography employing an immobilized Human Resistin matrix.

Sandwich ELISA:
To detect Human Resistin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Resistin (500-P183Bt) as a detection antibody, allows the detection of at least 2000–4000 ng/well of Recombinant Human Resistin.

Western Blot:
To detect Human Resistin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Resistin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin.
Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine specifically secreted by adipocytes. While its function is not fully understood, resistin appears to play key paracrine roles and has been linked to type 2 diabetes.
Studies show that in obese, diabetic mice, serum resistin levels are increased, antagonizing insulin action. Additional studies demonstrate that resistin induces insulin resistance and glucose intolerance in mice.
Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Coactivators CBP and p300, which have histone acetyltransferase (HAT) activity, acetylate histones at the resistin promoter, activating chromatin for transcription.
Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.