Thermo Fisher Scientific Resistin Polyclonal Antibody, PeproTech
Murine Resistin을 검출하기 위한 고순도 항원 친화 정제 폴리클로날 항체. Western Blot 및 ELISA에 최적화되어 있으며, Goat 유래로 높은 특이성과 감도를 제공. 비결합(Unconjugated) 형태로 -20°C에서 안정적 보관 가능.
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Thermo Fisher Scientific Resistin Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine Resistin |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929683 |
Product Specific Information
AA Sequence of recombinant protein:
SSMPLCPIDE AIDKKIKQDF NSLFPNAIKN IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI DWTAARCCKL QVAS
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine Resistin. Anti-Murine Resistin-specific antibody was purified by affinity chromatography using an immobilized Murine Resistin matrix.
Sandwich ELISA:
To detect Murine Resistin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antibody, in conjunction with PeproTech Biotinylated Anti-Murine Resistin (500-P182GBt), allows detection of at least 0.2–0.4 ng/well of Recombinant Murine Resistin.
Western Blot:
To detect Murine Resistin by Western Blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Murine Resistin is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Adipose tissue serves as an energy reserve and produces numerous physiologically important proteins such as LPL, leptin, Acrp30, perilipin, and resistin.
Resistin (also known as Adipocyte Secreted Factor, ADSF, and FIZZ3) is a cytokine secreted specifically by adipocytes. It is implicated in type 2 diabetes, insulin resistance, and glucose intolerance in mice.
Resistin gene expression requires C/EBP alpha and is modulated by coactivators CBP and p300 through histone acetylation at the resistin promoter. PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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