Thermo Fisher Scientific Calretinin Monoclonal Antibody (SP13)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:30

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP13

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Protein A

Storage buffer

PBS, pH 7.20, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981149

Product Specific Information

This antibody is predicted to react with bovine, chicken and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is mesothelioma.

Target Information

Calretinin is encoded by the CALB2 gene located on chromosome 16. It is a troponin C superfamily protein having six EF-hand structural motifs. The EF motif allows the protein to bind calcium, and function in calcium signaling, calcium buffering, and modulating neuronal excitability. Calretinin is expressed in the mesothelium, mast cells, some neural cells, hair follicles, and fat cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.