Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (DC10)
Cytokeratin 18 단백질을 검출하기 위한 Mouse Monoclonal Antibody (Clone DC10). 다양한 종(인간, 마우스, 영장류)에 반응하며 WB, IHC, ICC 등 다중 응용 가능. Protein A/G 정제, PBS+BSA 보존, 연구용 전용 시약.
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Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (DC10)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 13 publications |
| Immunohistochemistry (IHC) | - | View 12 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | - | View 1 publication |
| ELISA | - | View 1 publication |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DC10 |
| Immunogen | Human breast cancer PMC42 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979639 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast carcinoma or skin tissue.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) is a low molecular weight acidic keratin that pairs with cytokeratin 8.
It is expressed in epithelial tissues of the gastrointestinal, respiratory, and urogenital tracts, as well as endocrine/exocrine and mesothelial cells.
Cytokeratin 18 is prevalent in non-squamous epithelia and adenocarcinomas, but absent in squamous cell carcinomas.
Hepatocellular carcinomas express only cytokeratins 8 and 18.
Mutations in the cytokeratin 18 gene are associated with cryptogenic cirrhosis.
Two transcript variants encoding the same protein are known.
Cytokeratin subtype expression patterns aid in distinguishing epithelial malignancies and are valuable in immunohistochemistry, cytopathology, and flow cytometry assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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