Thermo Fisher Scientific Synaptophysin Monoclonal Antibody (SP11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 28 publications 28 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP11

Immunogen

Synthetic peptide corresponding to C-terminus of human synaptophysin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Synaptophysin,uniProtId:P08247-1,ncbiNodeId:9606,antigenRange:313,antigenLength:313,antigenImageFileName:MA1-39558_Synaptophysin_P08247-1_Rabbit.svg,antigenImageFileNamePDP:MA1-39558_Synaptophysin_P08247-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant/TBS, pH 7.5, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_10984801

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is pancreas or neuroendocrine tumor.

Target Information

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.