Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (SP1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25

View 27 publications 27 publications

Immunohistochemistry (IHC)

-

View 195 publications 195 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 22 publications 22 publications

Immunocytochemistry (ICC/IF)

1:25-1:250

View 6 publications 6 publications

Flow Cytometry (Flow)

1:200

View 1 publication 1 publication

ChIP assay (ChIP)

5 µL/10^6 cells

-

Dot blot (DB)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat, Sheep, Virus

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP1

Immunogen

Synthetic peptide derived from C-terminus of human estrogen receptor alpha if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Estrogen Receptor alpha,uniProtId:P03372-1,ncbiNodeId:9606,antigenRange:595,antigenLength:595,antigenImageFileName:MA1-39540_Estrogen_Receptor_alpha_P03372-1_Rabbit.svg,antigenImageFileNamePDP:MA1-39540_Estrogen_Receptor_alpha_P03372-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.014 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979688

Product Specific Information

This antibody is predicted to react with porcine based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.