Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.6), Functional Grade, eBioscience
인간 CD28 항원을 인식하는 단클론 항체로, T세포 활성화 및 공자극 연구에 적합합니다. Flow cytometry, Western blot, 중화 및 기능성 분석에 사용됩니다. 고순도(>90%) 항체이며, 1 mg/mL 농도로 제공되고 4°C에서 보관합니다.
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5개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.6), Functional Grade, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 1 µg/test | 8 publications |
| Neutralization (Neu) | Assay-Dependent | 1 publication |
| Functional Assay (Functional) | Assay-Dependent | 10 publications |
| T-Cell Activation (TCA) | - | 3 publications |
| In vitro Assay (IV) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD28.6 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865684 |
Product Specific Information
Description:
The CD28.6 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2), acting as a potent costimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases cytotoxicity of CD3-activated T cells. The CD28.6 antibody has been reported for use in immunoblotting (WB), neutralization studies, and co-stimulation assays related to IL-2 production.
Applications Reported:
Reported for use in flow cytometric analysis and immunoblotting (WB). The recommended titer for WB is approximately 1 µg/mL. CD28.6 has also been reported in in vitro functional assays, exhibiting both blocking and co-stimulatory activities.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Can be used at ≤1 µg per test (defined as the amount of antibody staining a sample in 100 µL). Cell numbers may range from 10⁵ to 10⁸ per test. It is recommended to titrate the antibody for optimal performance.
Storage and Handling:
Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell-specific surface glycoprotein, part of the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and functions as a critical co-stimulatory receptor. CD28 provides the essential second activation signal by binding CD80 and CD86 on antigen-presenting cells. It prevents T cells from entering an anergic state or undergoing premature apoptosis. In murine lymphoid organs and blood, all CD4+ and CD8+ T cells express CD28. Expression is highest on immature thymocytes (CD3-, CD8+, CD4+8+, and CD4-8- subsets). CD28 dysfunction is associated with diseases such as mycosis fungoides and Sézary’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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