Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugates

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 563, 661, 737, 805
• Brilliant Violet 421, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 585, 610-70S, 660-120S, 760
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

Product Specific Information

Description:
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as activated mature T and B lymphocytes. CD25 binds IL-2 with low affinity alone but forms a high-affinity IL-2 receptor complex with CD122 (IL-2Rβ) and CD132 (common γ chain). The PC61.5 antibody inhibits IL-2 binding to both high- and low-affinity receptors, affecting lymphocyte differentiation, activation, and proliferation.

Applications Reported:

• Flow cytometric analysis
• In vitro blocking of mouse CD25 (IL-2Rα, p55)

Applications Tested:

• Tested on mouse spleen cells and Con A-stimulated splenocyte cultures
• Recommended use ≤ 0.5 µg/test (in 100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test
• Optimize concentration for best performance

Storage and Handling:

• Use in sterile environment
• Store at 4°C
• 0.2 µm post-manufacturing filtered

Purity and Quality:

• Purity: >90% (SDS-PAGE)
• Endotoxin level: <0.001 ng/µg antibody (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. The IL2 receptor complex (IL2RA, IL2RB, IL2RG) forms a high-affinity binding site for IL2. IL2RA homodimers yield low-affinity receptors, while IL2RB homodimers yield medium-affinity receptors. IL2 expression in mature thymocytes is monoallelic, regulating precise gene expression. IL2 acts as a growth and differentiation factor, stimulating B cell proliferation, NK cell activity, and inhibiting granulocyte macrophage colony formation. Disruption of IL2RA in mice causes immune dysregulation resembling ulcerative colitis, indicating its crucial role in immune response. Mutations in IL2RA are linked to interleukin-2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.