Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscience
CD28.2 단클론 항체는 인간 CD28 분자를 인식하며 T 세포 활성화 및 공동자극 연구에 적합합니다. Flow cytometry 및 in vitro functional assay에 최적화되어 있으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 고순도(>90%) 및 낮은 엔도톡신 수준을 보장합니다.
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Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 4 |
| Flow Cytometry (Flow) | 1 µg/test | 50 |
| ELISA | - | 2 |
| Functional Assay | Assay-Dependent | 34 |
| T-Cell Activation (TCA) | - | 4 |
| Affinity Purification (AP) | - | 2 |
| In vitro Assay (IV) | - | 6 |
| Miscellaneous PubMed | - | 9 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Fruit fly, Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD28.2 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865687 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 615, 737
- Brilliant Violet 480, 650, 786
- eFluor 450
- FITC
- NovaFluor Blue 610-70S, 660-120S, 690
- NovaFluor Red 710, 725, 755
- NovaFluor Yellow 660, 755
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645
- Custom conjugation available upon request
Product Specific Information
The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2), serving as a potent co-stimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases cytotoxicity of CD3-activated T cells.
Applications Reported:
- Flow cytometric analysis
- Costimulation of T cells in in vitro functional assays
Applications Tested:
- Tested by flow cytometric analysis of normal human peripheral blood cells
- Recommended ≤1 µg per test (100 µL final volume)
- Cell number range: 10⁵–10⁸ cells/test
- Titrate for optimal performance
Storage and Handling:
- Use in sterile environment
- 0.2 µm post-manufacturing filtered
Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
Aggregation: <10% (HPLC)
Target Information
CD28 antigen is a 44 kDa disulfide-linked homodimeric T-cell surface glycoprotein belonging to the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and acts as a critical co-stimulatory receptor, providing the second activation signal by binding CD80 and CD86 on antigen-presenting cells. CD28 prevents T cells from entering an anergic state or premature apoptosis. Expression varies across T-cell maturation stages, being highest on immature thymocytes. Dysfunction of CD28 is associated with diseases such as mycosis fungoides and Sezary’s Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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