Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Preparation:
Produced from sera of goats immunized with highly pure recombinant Murine SDF-1alpha (CXCL12). Anti-Murine SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography employing an immobilized Murine SDF-1alpha (CXCL12) matrix.

Sandwich ELISA:
To detect Murine SDF-1alpha (CXCL12) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. When used with PeproTech Biotinylated Anti-Murine SDF-1alpha (CXCL12) (500-P164GBt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of recombinant Murine SDF-1alpha (CXCL12).

Western Blot:
To detect Murine SDF-1alpha (CXCL12) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for recombinant Murine SDF-1alpha (CXCL12) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in metastasis of cancers such as breast cancer. Mutations in this gene are associated with resistance to HIV-1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.