Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech
CXCL12 alpha(SDF-1 alpha)에 특이적인 염소 폴리클로날 항체로, 마우스 시료에 반응합니다. Western blot, ELISA, 면역염색 등에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며 연구용으로 적합합니다.
- 카탈로그번호
- 500-P164G-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 3 publications |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| In vitro Assay (IV) | – | View 2 publications |
| Immunostaining (IS) | – | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine SDF-1alpha (CXCL12) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929581 |
Product Specific Information
AA Sequence of recombinant protein:
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
Preparation:
Produced from sera of goats immunized with highly pure recombinant Murine SDF-1alpha (CXCL12). Anti-Murine SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography employing an immobilized Murine SDF-1alpha (CXCL12) matrix.
Sandwich ELISA:
To detect Murine SDF-1alpha (CXCL12) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. When used with PeproTech Biotinylated Anti-Murine SDF-1alpha (CXCL12) (500-P164GBt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of recombinant Murine SDF-1alpha (CXCL12).
Western Blot:
To detect Murine SDF-1alpha (CXCL12) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for recombinant Murine SDF-1alpha (CXCL12) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in metastasis of cancers such as breast cancer. Mutations in this gene are associated with resistance to HIV-1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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