Thermo Fisher Scientific BAFF Polyclonal Antibody, PeproTech
Human BAFF 단백질을 인식하는 Goat Polyclonal Antibody로, Western Blot 및 ELISA에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 민감도를 제공. Lyophilized 형태로 -20°C에서 안정적 보관 가능.
- 카탈로그번호
- 500-P163G-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific BAFF Polyclonal Antibody, PeproTech
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA | 0.5–2.0 µg/mL | – |
| Immunostaining (IS) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human BAFF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929575 |
Product Specific Information
AA Sequence of recombinant protein:
AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography employing an immobilized Human BAFF matrix.
Sandwich ELISA:
To detect Human BAFF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BAFF (500-P163GBt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human BAFF.
Western Blot:
To detect Human BAFF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human BAFF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
BAFF is a membrane-bound cytokine expressed by B cell lineage cells. The soluble form binds to receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF acts as a potent B cell activator, important for proliferation and differentiation of B cells. It is also expressed by T lymphocytes and promotes T cell activation and survival. Isoforms representing alternatively spliced and glycosylated variants have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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