Thermo Fisher Scientific BAFF Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific BAFF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.25–1.0 µg/mL
  • Sandwich ELISA: 검출 한계 0.2–0.4 ng/well (100 µL/well 사용 시)

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hBAFF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BAFF (500-P163G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hBAFF.

Western Blot:
To detect hBAFF by Western blot analysis, use at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBAFF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P163GBT-1MG is provided as 2 × 500 µg.

Target Information

BAFF is a membrane-bound cytokine expressed by B cell lineage cells. The soluble form binds to receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, critical for proliferation and differentiation of B cells. It is also expressed by T lymphocytes and promotes T cell activation and survival. Isoforms representing alternatively spliced and glycosylated variants have been identified.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일명: 500-P163GBT-500UG_BAFF_BLyS_Q9Y275-1_Goat.svg, 500-P163GBT-500UG_BAFF_BLyS_Q9Y275-1_Goat_PDP.jpeg)