Thermo Fisher Scientific CD267 (TACI) Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
SGLGRSRRGG RSRVDQEERF PQGLWTGVAM RSCPEEQYWD PLLGTCMSCK TICNHQSQRT CAAFCRSLSC RKEQGKFYDH LLRDCISCAS ICGQHPKQCA YFCENKLRSP VNLPPELRRQ RSGEVENNSD NSGRYQGLEH RGSEASPALP GLKLSADQV.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human TACI. Anti-Human TACI-specific antibody was purified by affinity chromatography employing an immobilized Human TACI matrix.

Sandwich ELISA:
To detect Human TACI by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TACI (500-P166GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human TACI.

Western Blot:
To detect Human TACI by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TACI is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL-1/THANK/zTNF4 and April/TALL-2, respectively.
BAFF acts as a B cell activator, inducing B cell proliferation and immunoglobulin secretion, while April regulates immunological and non-immunological cell growth.
Three receptors — BCMA, TACI, and BAFF-R — for BAFF and April have been identified. TACI, like BCMA, binds both BAFF and April. TACI and its ligands regulate humoral immune responses, activate NF-kappa-B and c-jun N-terminal kinase, and are involved in B cell-associated autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.