Thermo Fisher Scientific CD267 (TACI) Polyclonal Antibody, PeproTech
Human TACI 단백질을 특이적으로 인식하는 염소 유래 Polyclonal 항체입니다. Western blot 및 ELISA에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human TACI |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929588 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
SGLGRSRRGG RSRVDQEERF PQGLWTGVAM RSCPEEQYWD PLLGTCMSCK TICNHQSQRT CAAFCRSLSC RKEQGKFYDH LLRDCISCAS ICGQHPKQCA YFCENKLRSP VNLPPELRRQ RSGEVENNSD NSGRYQGLEH RGSEASPALP GLKLSADQV.
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human TACI. Anti-Human TACI-specific antibody was purified by affinity chromatography employing an immobilized Human TACI matrix.
Sandwich ELISA:
To detect Human TACI by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TACI (500-P166GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human TACI.
Western Blot:
To detect Human TACI by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TACI is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL-1/THANK/zTNF4 and April/TALL-2, respectively.
BAFF acts as a B cell activator, inducing B cell proliferation and immunoglobulin secretion, while April regulates immunological and non-immunological cell growth.
Three receptors — BCMA, TACI, and BAFF-R — for BAFF and April have been identified. TACI, like BCMA, binds both BAFF and April. TACI and its ligands regulate humoral immune responses, activate NF-kappa-B and c-jun N-terminal kinase, and are involved in B cell-associated autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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