Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 510, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.8 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Brilliant Violet, eFluor, FITC, PE, PE-Cyanine, PerCP-Cyanine, Super Bright 시리즈
• NovaFluor Blue, Red, Violet, Yellow 시리즈
• Request custom conjugation available

Product Specific Information

Description:
The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all developmental stages except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, and MHC class II, CD19 forms a multimolecular complex associated with the BCR, mediating signaling, phosphorylation, calcium flux, and B cell proliferation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.8 µg/test (100 µL final volume). Optimize titration for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Technology:
NovaFluor conjugates use Phiton™ technology with engineered nucleic acid dye structures for optimized spectral performance and minimal spillover.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

CD19:
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells and myeloid leukemia cells. It serves as a key regulator of B cell receptor (BCR) signaling and proliferation. CD19 mutations can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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