Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 510

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.8 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series
• PE, PE-Cyanine series
• Super Bright series
• NovaFluor Blue, Red, Violet, Yellow variants
• Custom conjugation available upon request

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except terminally differentiated plasma cells. CD19 forms a complex with CD21, CD81, and MHC class II that associates with the BCR, inducing tyrosine phosphorylation, calcium flux, and B-cell proliferation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes at ≤0.8 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability testing.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates utilize Phiton™ technology, engineered for optimized fluorescence signatures with minimal spillover and spread.
Excitation: 496 nm; Emission: 511 nm; Laser: 488 nm (Blue)

Target Information

CD19 is a transmembrane glycoprotein of the immunoglobulin superfamily, expressed on all peripheral B cells and B-cell precursor leukemias. It acts as a co-receptor with BCR, amplifying antigen receptor signaling and regulating B-cell activation and proliferation.
Mutations in CD19 cause hypogammaglobulinemia, while overexpression leads to B-cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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