Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 555, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.4 µg/test

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395–805, Brilliant Violet 421–786, eFluor 450–660, FITC, Functional Grade, NovaFluor Blue 510–760, NovaFluor Red 710, NovaFluor Yellow 570–755, PE, PE-Cyanine5–7, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright 436–780, Custom conjugation available.

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells at all developmental stages except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, and MHC class II, associating with the BCR to regulate B cell activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.4 µg/test (100 µL final volume). Optimal titration is recommended.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability detection.
• Use CellBlox Blocking Buffer (Cat. B001T06F01) when staining with NovaFluor conjugates. Add 5 µL per sample, or 5 µL per 100 µL for single-color controls.
• Excitation: 496 nm; Emission: 555 nm; Laser: 488 nm (Blue)

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and certain myeloid leukemia cells. CD19 functions as a signal-amplifying coreceptor for the B cell receptor (BCR), modulating antigen sensitivity and B cell activation. Mutations in CD19 can lead to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures.