Thermo Fisher Scientific RIG-I Polyclonal Antibody

Thermo Fisher Scientific RIG-I Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

A suggested positive control is C2C12 cell lysate.

Target Information

Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor (PRR) involved in the recognition of viral double-stranded RNA (dsRNA). Along with MDA5, RIG-I detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases that perform overlapping and distinct roles.
RIG-I is activated by short dsRNAs lacking a 5′-triphosphate end, whereas MDA5 is activated by long dsRNAs. Upon activation, both proteins signal through IPS-1 to activate transcription factors NF-κB and IRF-3, ultimately inducing apoptosis, cytokine signaling, and inflammation.
RIG-I is essential for signaling by influenza A, influenza B, human respiratory syncytial virus, paramyxoviruses, Japanese encephalitis virus, and West Nile virus. MicroRNA-146a has been implicated in feedback inhibition of RIG-I-dependent antiviral response by negatively regulating RIG-I targets TRAF6, IRAK1, and IRAK2. Recent evidence also suggests RIG-I involvement in cytosolic DNA detection through RNA polymerase III activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.