Thermo Fisher Scientific RIG-I Polyclonal Antibody
RIG-I 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 액상 형태, 1 mg/mL 농도, PBS 버퍼 및 0.02% sodium azide 포함. 바이러스 dsRNA 인식 연구에 적합.
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Thermo Fisher Scientific RIG-I Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 5 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human GST-tagged RIG-I protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months; for long-term storage store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152363 |
Product Specific Information
A suggested positive control is C2C12 cell lysate.
Target Information
Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor (PRR) involved in the recognition of viral double-stranded RNA (dsRNA). Along with MDA5, RIG-I detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases that perform overlapping and distinct roles.
RIG-I is activated by short dsRNAs lacking a 5′-triphosphate end, whereas MDA5 is activated by long dsRNAs. Upon activation, both proteins signal through IPS-1 to activate transcription factors NF-κB and IRF-3, ultimately inducing apoptosis, cytokine signaling, and inflammation.
RIG-I is essential for signaling by influenza A, influenza B, human respiratory syncytial virus, paramyxoviruses, Japanese encephalitis virus, and West Nile virus. MicroRNA-146a has been implicated in feedback inhibition of RIG-I-dependent antiviral response by negatively regulating RIG-I targets TRAF6, IRAK1, and IRAK2. Recent evidence also suggests RIG-I involvement in cytosolic DNA detection through RNA polymerase III activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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