Thermo Fisher Scientific CLCN3 Monoclonal Antibody (N258/5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N258/5

Immunogen

Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CLCN3,uniProtId:P51792-1,ncbiNodeId:10114,antigenRange:98-115,antigenLength:818,antigenImageFileName:MA5-27709_CLCN3_P51792-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27709_CLCN3_P51792-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735150

Product Specific Information

1 µg/mL of MA5-27709 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).

This antibody was formerly sold as clone S258-5.

Target Information

Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.