
Thermo Fisher Scientific DYKDDDDK Tag Recombinant Superclonal Antibody (20H18L16, 20H1L23, 8H2L5, 8H8L17)
Recombinant Superclonal™ 항체로 DYKDDDDK(FALG) 태그 단백질 검출에 최적화. WB, IHC, ICC, IP 등 다양한 응용 가능. 높은 재현성과 감도로 저발현 단백질 분석에 적합. 4종 클론 혼합으로 폴리클로날 감도와 모노클로날 특이성을 모두 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 6 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 7 publications |
| Immunoprecipitation (IP) | 1 µg/mL | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | C. elegans, Human, Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Clone | 20H18L16, 20H1L23, 8H2L5, 8H8L17 |
| Immunogen | Synthetic peptide (DYKDDDDK) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2610628 |
Product Specific Information
This antibody consists of four clones: 20H18L16 and 20H1L23 from the N-terminal, and 8H2L5 and 8H8L17 from the C-terminal.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are composed of a mixture of recombinant antibodies co-expressed from a library of heavy and light chains, combining the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies, while maintaining lot-to-lot consistency.
These antibodies recognize multiple epitope sites on the target, providing higher detection sensitivity for low-abundance targets. Each lot contains the same population of heavy and light chains, eliminating biological variability typical of polyclonal production.
Note: Formerly called “Recombinant Polyclonal Antibody”, now rebranded as “Recombinant Superclonal™ Antibody”. The physical product and performance remain unchanged.
Target Information
DYKDDDDK tag (also known as FLAG tag) is a short peptide sequence used as a protein tag to facilitate purification, detection, and localization.
The tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) can be fused to either terminus of a protein of interest, enabling easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely applied in:
- Protein-protein interaction studies
- Protein expression analysis
- Protein localization research
The FLAG tag is optimized for hydrophilicity, minimizing potential interference with protein folding or activity.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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