Thermo Fisher Scientific DYKDDDDK Tag Recombinant Superclonal Antibody (20H18L16, 20H1L23, 8H2L5, 8H8L17)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 7 publications 7 publications

Immunoprecipitation (IP)

1 µg/mL

-

Product Specifications

Species Reactivity

Tag

Published species

C. elegans, Human, Tag

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

20H18L16, 20H1L23, 8H2L5, 8H8L17

Immunogen

Synthetic peptide (DYKDDDDK)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2610628

Product Specific Information

This antibody consists of four clones viz. 20H18L16 and 20H1L23 from the N terminal and 8H2L5 and 8H8L17 from the C terminal.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a mixture of recombinant antibodies co-expressed from a library of heavy and light chains, providing the best of both worlds the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets the exact population of the heavy and light chains in a recombinant rabbit Superclonal™ antibody can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.