Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI2D2), TrueMAB
MAP2K1(MEK1) 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC에 적합합니다. Human, Mouse, Rat, Dog 시료에 반응하며, 고순도 친화 크로마토그래피 정제 제품입니다. 동결건조 형태로 -20°C 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D2 |
| Immunogen | Full length human recombinant protein of human MAP2K1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) is a dual-specificity protein kinase involved in MAPK signaling cascades that regulate cell growth and differentiation. It is activated by various growth factors, cytokines, and membrane depolarization. Phosphorylation at serine residues 218 and 222 by Raf1 activates MEK1. It plays roles in stress response, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 are associated with cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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