Thermo Fisher Scientific RAB21 Monoclonal Antibody (OTI6E12), TrueMAB
RAB21 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC에 적합합니다. Human, Mouse, Rat, Dog 반응성을 가지며, Lyophilized 형태로 제공됩니다. 고순도 친화 크로마토그래피 정제, 연구용 전용 시약입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6E12 |
| Immunogen | Full length human recombinant protein of human RAB21 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RAB21 is a member of the small GTP-binding protein family within the Ras superfamily, involved in intracellular vesicular trafficking.
In non-polarized Caco-2 cells, RAB21 shows ER-like localization, whereas in polarized cells it localizes to apical vesicles.
It associates with integrin subunits and facilitates receptor entry via RAB5/RAB21 endosomes.
The GTPase-activating protein p120RasGAP regulates cell motility by modulating integrin recycling through competition with RAB21 for binding sites on the α-tail of endocytosed integrins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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