Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), PE, eBioscience

Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), PE, eBioscience™

Applications

• Flow Cytometry (Flow): Tested at 5 µL (1 µg)/test
• Publications: View 5 publications

Product Specifications

Additional Formats

• APC
• Functional Grade
• NovaFluor Red 710
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 42D1 recognizes the CD85d (ILT4/LIR-2) protein, a member of the ILT/LIR/MIR receptor family.
CD85d is a 110 kDa transmembrane receptor with three Ig-like domains and a long cytoplasmic domain containing ITIMs.
Expression is confined to monocytes, macrophages, and dendritic cells.
Its ligand includes non-classical class I HLA-G, -B, and G1, and upon ligand binding, SHP-1 interacts with ITIMs.
Interaction between HLA-G and ILT4/CD85d contributes to tolerance during fetal implantation and may play a role in organ allograft rejection.

Crosslinking with antibody 42D1 inhibits calcium flux and enhances HLA-G tetramer staining, restoring stimulatory activity of tolerogenic DCs.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (1 µg) per test (100 µL final volume).
Cell count range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LILRB2 (ILT4) gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at chromosomal region 19q13.4.
This receptor contains multiple extracellular Ig domains, a transmembrane region, and cytoplasmic ITIMs.
Expressed on immune cells, it binds MHC class I molecules to transmit inhibitory signals that regulate immune responses, inflammation, and autoreactivity.
Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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