
Thermo Fisher Scientific CD85h (ILT1) Monoclonal Antibody (135.4), PE, eBioscience
인간 CD85h(ILT1)를 인식하는 단클론 항체로, PE 형광표지되어 Flow Cytometry 분석에 최적화됨. 모노사이트, 대식세포, DC 등에서 ILT1 발현 검출 가능. 4°C 보관, 동결 금지. 연구용 전용.
- 카탈로그번호
- 12-5119-42
- 판매단위
- pk
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Thermo Fisher Scientific CD85h (ILT1) Monoclonal Antibody (135.4), PE, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
Applications Reported: Flow cytometric analysis of normal human peripheral blood cells
Applications Tested: Pre-titrated for use at 5 µL (0.5 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 135.4 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10671015 |
Product Specific Information
The monoclonal antibody 135.4 recognizes ILT1 (CD85h, LIR7, LILRA2), a member of the ILT/LIR/MIR receptor family.
ILT1 has a short cytoplasmic domain that associates with the Fc receptor gamma chain, producing a stimulatory signal.
ILT1 may act as a receptor for MHC Class I antigens and is mainly expressed on monocytes, macrophages, dendritic cells, and weakly on granulocytes.
It plays a role in TH1/Th2 balance and TLR signaling.
Crosslinking of this antibody can induce calcium influx.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Leukocyte Ig-like receptors (LIRs) are immunoreceptors expressed predominantly on monocytes and B cells, and at lower levels on dendritic cells and NK cells.
Subfamily B members (e.g., LILRB1) have inhibitory functions, while subfamily A members (e.g., LILRA3) with short cytoplasmic domains and charged transmembrane regions may initiate stimulatory cascades.
LILRA3 lacks a transmembrane region and is presumed to be soluble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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