Thermo Fisher Scientific CX3CR1 Monoclonal Antibody (2A9-1), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CX3CR1 Monoclonal Antibody (2A9-1), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 3 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• Biotin
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 702
• Request custom conjugation

Product Specific Information

This 2A9-1 monoclonal antibody reacts with human CX3CR1, the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven-transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (CD8+, CD4+, γ/δ), and monocytes. It is also found on osteoclast precursors and microglia, but not on B cells or granulocytes.

Fractalkine and CX3CR1 mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. CX3CR1 expression correlates with high intracellular perforin and granzyme B levels.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry on normal human peripheral blood cells
  • Use at 5 µL (0.125 µg) per test (100 µL final volume)
  • Applicable for 10⁵–10⁸ cells/test

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples may be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CX3CR1 is a chemokine receptor acting as a coreceptor for HIV infection. Human, mouse, and rat CX3CR1 genes were cloned (V28, CMKBRL1, RBS11). The protein functions as a receptor for fractalkine and mediates leukocyte migration and adhesion. CX3CR1 is expressed in various human tissues and cell lines.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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