Thermo Fisher Scientific Nur77 Monoclonal Antibody (12.14), PE-Cyanine7, eBioscience
Mouse Nur77 단백질을 인식하는 12.14 클론의 단일클론 항체로, PE-Cyanine7 형광체가 결합된 제품입니다. Flow cytometry 및 intracellular staining에 최적화되어 있으며, 0.25 µg/test로 사용 가능합니다. 4°C 암소 보관, 광민감성 주의.
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Thermo Fisher Scientific Nur77 Monoclonal Antibody (12.14), PE-Cyanine7, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12.14 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811785 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 647
- eFluor 450
- PE
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
This 12.14 monoclonal antibody reacts with mouse Nur77 (NR4A1, TR3, NGFI-B, NAK1), an inducible orphan nuclear receptor expressed in thymocytes and T cell lines. Nur77 promotes apoptosis and plays a role in thymocyte negative selection. It is also critical for steroid biosynthesis in Leydig cells and dopamine-related effects. Interaction with FoxP3 in regulatory T cells has been reported, though not observed with this antibody.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis.
Applications Tested:
- Tested in intracellular staining of stimulated mouse thymocytes using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
- Recommended ≤0.25 µg per test (100 µL final volume).
- Cell number range: 10⁵–10⁸ cells/test.
- Antibody titration recommended for optimal performance.
Light Sensitivity:
- Protect vial and stained samples from light (photo-induced oxidation).
Fixation:
- Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark.
- Clone-specific performance should be empirically determined.
Excitation / Emission / Laser:
- Excitation: 488–561 nm
- Emission: 775 nm
- Laser: Blue, Green, Yellow-Green
Target Information
NR4A1 belongs to the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in various tissues including brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung, and prostate. It mediates apoptotic signaling and plays a role in hypothalamic-pituitary axis signaling. Translocation from nucleus to mitochondria induces apoptosis. Three transcript variants encode two isoforms; variant (3) or TRC beta differs from variant 1 with a shorter C-terminus. RXR acts as a partner for NR4A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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