Thermo Fisher Scientific MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), PE-Cyanine7, eBioscience
AF6-88.5.5.3 단클론 항체로서 마우스 H-2Kb MHC Class I 항원을 인식합니다. Flow Cytometry용으로 최적화된 PE-Cyanine7 형광 접합체입니다. 높은 특이성과 안정적인 형광 신호를 제공하며, 4°C 암조건에서 보관합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), PE-Cyanine7, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Related Publications: View 16 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF6-88.5.5.3 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573504 |
Additional Formats Available
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Custom conjugation available upon request
Product Specific Information
This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC Class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g., d, f, j, k, p, q, r, s, u, and v) has not been observed.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.25 µg/test (100 µL final volume, cell number 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.
Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
This protein is an MHC Class I alloantigen involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are essential tools in Immunopeptidomics, enabling identification and characterization of neoantigens through high-performance liquid chromatography coupled with tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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