Thermo Fisher Scientific HLA-A2 Monoclonal Antibody (BB7.2)

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• FITC
• PE
• Request custom conjugation

Product Specific Information

This product is a low-endotoxin, preservative-free formulation (endotoxin level <0.01 EU/µg).
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
A suggested positive control for immunohistochemical applications is tonsil.

Mouse anti-Human HLA-A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatibility antigen.

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (β2-microglobulin). The heavy chain is membrane-anchored and plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen.

HLA-A is expressed in nearly all nucleated cells. The heavy chain (~45 kDa) gene contains 8 exons:

1. Exon 1 – Leader peptide
2. Exons 2 & 3 – α1 and α2 domains (peptide binding)
3. Exon 4 – α3 domain
4. Exon 5 – Transmembrane region
5. Exons 6 & 7 – Cytoplasmic tail

Polymorphisms within exons 2 and 3 determine peptide-binding specificity. Typing of these polymorphisms is routinely performed for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are crucial in immunopeptidomics, enabling identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.