Thermo Fisher Scientific Desmoglein-3 Monoclonal Antibody (DSG3, 2838)
Desmoglein-3 단백질을 표적으로 하는 mouse monoclonal 항체. IHC(P) 및 Peptide Array에 사용 가능. 인간 시료 반응성, Protein A/G 정제, 4°C 보관. Pemphigus vulgaris 연구에 유용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DSG3, 2838 |
| Immunogen | Recombinant fragment (around aa 379–491) of human DSG3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominantly expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 is characterized as the autoantigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes loss of cell-cell adhesion, resulting in blister formation on the skin and oral mucosa, and histologically as suprabasilar acantholysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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