Thermo Fisher Scientific IL-6 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIL EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Rat IL-6. Anti-Rat IL-6-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat IL-6 by sandwich ELISA (100 µL/well antibody solution), use at 0.25–1.0 µg/mL.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-6 (500-P73G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat IL-6.

Western Blot:
For detection of Rat IL-6 by Western Blot, use at 0.1–0.2 µg/mL.
When used with compatible secondary reagents, detection limit is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P73GBT-1MG will be provided as 2 × 500 µg.

Target Information

IL-6 encodes a cytokine involved in inflammation and B cell maturation. The protein acts as an endogenous pyrogen that can induce fever in autoimmune diseases or infections.
It is mainly expressed at sites of acute and chronic inflammation, secreted into serum, and triggers a transcriptional inflammatory response via the interleukin 6 receptor alpha.
IL-6 is implicated in various inflammation-associated diseases, including diabetes mellitus susceptibility and systemic juvenile rheumatoid arthritis.
Elevated IL-6 levels are found in viral infections, including COVID-19 (SARS-CoV-2).
Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.