Thermo Fisher Scientific CXCL1 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Recommended for Sandwich ELISA using PeproTech Polyclonal Anti-Rat GRO/KC (500-P74) as capture antibody

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat GRO/KC (CXCL1). Anti-Rat GRO/KC (CXCL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat GRO/KC by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat GRO/KC (500-P74) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat GRO/KC.

Western Blot:
To detect Rat GRO/KC by Western Blot analysis, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, detection limit for Recombinant Rat GRO/KC is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Package:
500-P74BT-1MG will be provided as 2 × 500 µg.

Target Information

CXCL1, CXCL2, and CXCL3 (GRO alpha, GRO beta, GRO gamma) are members of the CXC chemokine subfamily.
Mature CXCL1/2/3 proteins bind with high affinity to IL-8 receptor type B and are potent neutrophil attractants and activators.
Processed forms GRO-alpha(4-73), GRO-alpha(5-73), and GRO-alpha(6-73) show ~30-fold higher chemotactic activity in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.