Thermo Fisher Scientific CXCL1 Polyclonal Antibody, PeproTech
Rat CXCL1(GRO/KC) 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot과 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 E.coli 유래 재조합 단백질을 사용해 제작되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
| Immunostaining (IS) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat GRO/KC (CXCL1) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930737 |
Product Specific Information
AA Sequence of recombinant protein:
APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat GRO/KC (CXCL1). Anti-Rat GRO/KC (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Rat GRO/KC (CXCL1) matrix.
Sandwich ELISA:
To detect Rat GRO/KC (CXCL1) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat GRO/KC (CXCL1) (500-P74Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat GRO/KC (CXCL1).
Western Blot:
To detect Rat GRO/KC by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Rat GRO/KC is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
CXCL1, CXCL2, and CXCL3—also known as GRO alpha, GRO beta (MIP-2 alpha), and GRO gamma (MIP-2 beta)—are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, processed forms GRO-alpha(4–73), GRO-alpha(5–73), and GRO-alpha(6–73) show a 30-fold higher chemotactic activity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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