Thermo Fisher Scientific HLA-DR Monoclonal Antibody (L243), PE-Cyanine5

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

4 µL/1x10^6 cells

-

Product Specifications

Species Reactivity

Dog, Human, Non-human primate

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

L243

Immunogen

Human B lymphocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-DR,uniProtId:P01903-1,ncbiNodeId:9606,antigenRange:1-254,antigenLength:254,antigenImageFileName:MA5-38756_HLA-DR_P01903-1_House_mouse.svg,antigenImageFileNamePDP:MA5-38756_HLA-DR_P01903-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898668

Product Specific Information

This antibody recognizes specifically an extracellular epitope on HLA-DR molecules, both peptide-loaded and empty.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.