Thermo Fisher Scientific ICAM-1 Monoclonal Antibody (3E2B)
Mouse ICAM-1(CD54)을 인식하는 단클론 항체로, WB, IHC, ELISA, FACS 등 다양한 응용에 사용 가능. Armenian hamster 유래 IgG 형식으로 Protein G 정제. 비보존 PBS 용액 형태로 제공되며, 내독소 ≤10 EU/mg.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:10–1:100 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | Assay-dependent | - |
| Neutralization (Neu) | Assay-dependent | View 2 publications |
| Inhibition Assays (IA) | Assay-dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E2B |
| Immunogen | Mouse ICAM-1 (CD54) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_223595 |
Product Specific Information
MA5405 targets CD54 in WB, ELISA, FACS, IA, IHC, ICC/IF, and Neu applications and shows reactivity with mouse samples.
The MA5405 immunogen is mouse ICAM-1 (CD54).
MA5405 detects CD54, which has a predicted molecular weight of approximately 56 kDa.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of ≤10 EU/mg.
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain.
It has 7 potential N-linked glycosylation sites and shares sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102).
ICAM-1 binds to integrins CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and serves as a receptor for Rhinovirus.
It is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils, with expression levels increasing during inflammation.
ICAM-1 is also found in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in patients with various inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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