Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (5A8E5), iFluor 647, THE
DYKDDDDK(FLAG) 태그 단백질 검출용 iFluor 647 결합 단일클론 항체. 높은 형광 안정성과 밝기 제공. ICC/IF 및 Flow Cytometry에 적합. 재조합 단백질의 N/C 말단 태그 분석에 활용 가능.
- 카탈로그번호
- A01811100
- 판매단위
- pk
카탈로그
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | 1–4 µg/mL | – |
| Flow Cytometry (Flow) | 1–4 µg/mL | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A8E5 |
| Immunogen | Synthetic peptide DYKDDDDK coupled to KLH |
| Conjugate | iFluor™ 647 |
| Excitation / Emission Max | 656 / 670 nm |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 10 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make an antibody concentration of 0.5 mg/mL.
The FLAG tag (DYKDDDDK) is a short peptide sequence that can be added to a target protein using recombinant DNA technology. It can be fused to either the N-terminus or C-terminus of the protein to facilitate detection and purification.
THE™ DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse is THE™ DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3–6.
It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye, serving as an excellent alternative to Alexa Fluor 647.
Target Information
DYKDDDDK tag, also known as FLAG tag, is an eight-amino-acid sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) commonly used for protein purification, detection, and localization.
It is often fused to the N- or C-terminus of a protein of interest, enabling easy identification and isolation using anti-FLAG antibodies.
The FLAG tag is hydrophilic, minimizing the risk of protein denaturation or inactivation.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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