Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (5A8E5), iFluor 647, THE

Applications

Product Specifications

Additional Formats Available

• Unconjugated
• Biotin
• FITC
• HRP
• iFluor 488
• iFluor 555
• Request custom conjugation

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make an antibody concentration of 0.5 mg/mL.
The FLAG tag (DYKDDDDK) is a short peptide sequence that can be added to a target protein using recombinant DNA technology. It can be fused to either the N-terminus or C-terminus of the protein to facilitate detection and purification.

THE™ DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse is THE™ DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3–6.
It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye, serving as an excellent alternative to Alexa Fluor 647.

Target Information

DYKDDDDK tag, also known as FLAG tag, is an eight-amino-acid sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) commonly used for protein purification, detection, and localization.
It is often fused to the N- or C-terminus of a protein of interest, enabling easy identification and isolation using anti-FLAG antibodies.
The FLAG tag is hydrophilic, minimizing the risk of protein denaturation or inactivation.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.