Thermo Fisher Scientific GLP1R Monoclonal Antibody (1F1E7)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

4 µg/mL

-

Flow Cytometry (Flow)

1-5 µg/mL

-

ELISA (ELISA)

0.1 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1F1E7

Immunogen

a plasmid DNA encoding a full length of human GLP1R (Swiss prot: P43220)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning.
GenScript GLP1R Antibody, mAb, Mouse is produced from a hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from a mouse immunized with a plasmid DNA encoding a full length of human GLP1R (Swiss prot: P43220).

Target Information

Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.