Thermo Fisher Scientific MARK3 Monoclonal Antibody (OTI3F6), TrueMAB
Human MARK3 단백질(aa 473-729)을 항원으로 한 Mouse IgG1 단클론 항체. Western blot 및 IHC(P) 실험에 적합. 무보존제, 동결건조 형태로 제공되며 재구성 후 1 mg/mL 농도. 세포 극성 및 MAP 인산화 연구용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 473–729 of human MARK3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MARK3 is a kinase involved in regulating cell polarity through phosphorylation of microtubule-associated proteins (MAPs). Activated MARK3 phosphorylates tau, MAP2, and MAP4 proteins. Multiple transcript variants encoding different isoforms of MARK3 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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