Thermo Fisher Scientific GATA-3 (Center) (Breast and Urothelial Marker) Monoclonal Antibody (GATA3/6664)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Antibody stability: 24 months
• Positive Control: Human breast or urothelial carcinoma tissue
• Cellular Localization: Nuclear

Specificity Comments:
GATA-3 (GATA binding protein 3) is a 50 kDa nuclear transcription factor involved in development and maintenance of multiple human tissues including hematopoietic cells, skin, kidney, mammary gland, and CNS. It plays a key role in luminal cell differentiation in the mammary gland and regulates genes associated with breast cancer proliferation. GATA-3 expression correlates with estrogen receptor-alpha (ER) expression in breast cancer and serves as a novel marker for bladder cancer, staining 67% of urothelial carcinomas but not prostate or renal carcinomas.

Target Information

The genes for the four subunits of the T-cell antigen receptor (α, β, γ, δ) are regulated by distinct enhancers and their enhancer-binding proteins. GATA3 binds to enhancer elements of all four TCR genes, acting as a zinc finger enhancer-binding protein. GATA3 mRNA is expressed in T cells but not in B cells or macrophages, indicating its role in T-lymphocyte lineage differentiation and TCR gene activation. The human GATA3 gene comprises six exons over 17 kb, with two zinc fingers encoded by separate exons highly conserved with GATA1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.